The widespread screening by measurement of serum prostatic specific antigen (PSA) and needle core biopsy allows more and more organ-confined prostate cancer being diagnosed. Orchiectomy as a treatment for prostate cancer almost completely disappears due to the availability of effective androgen ablative therapy. It is rarely necessary for the urologists to rely on intraoperative FS diagnosis for surgical treatment. However, there are still situations that FS is indicated for guidance of the surgical intervention as listed below: 1. Evaluation of surgical margin status during radical prostatectomy. 2. Evaluation of metastatic status of pelvic lymph nodes during radical prostatectomy. 3. Intraoperative diagnosis of adenocarcinoma in prostate from organ donors. 4. Intraoperative diagnosis of prostatic transurethral resection specimens, or simple prostatectomy specimens for nodular hyperplasia.