This study evaluated the effects of exercise-induced muscle damage (EIMD) on fixed-load cycling and 5-min time-trial performance. Seven recreational athletes performed two submaximal fixed-load exercise bouts followed by a 5-min time-trial before, 48 and 168 h following 100 counter-movement jumps. Measurements of $$ \dot{V}{\text{O}}_{2} , $$ heart rate, RER and blood lactate concentration remained unchanged during the fixed-load bouts following jumping exercise. However, $$ \dot{V}_{\text{E}} $$ and $$ \dot{V}_{\text{E}} /\dot{V}{\text{O}}_{2} $$ increased (P < 0.05) at 48 h. RPE values were higher at 48 h as were the ratio of RPE:HR and RPE: $$ \dot{V}{\text{O}}_{2} $$ (P < 0.05). In the time-trial, mean $$ \dot{V}{\text{O}}_{2} , $$ peak power output, mean power output, distance covered and post exercise blood lactate were lower at 48 h (P < 0.05). RPE remained unchanged between trials. These findings indicate that the ventilatory equivalent for oxygen and perceived exertion at submaximal work rates are increased 48 h following eccentric exercise. Furthermore, EIMD increases perceived exertion and impairs performance during a 5-min all-out effort.