Abstract. Sedums are used as groundcover, in rock gardens and flower borders, and for greening the top floor of buildings, cottages, and thatched roofs. In this study, Agrobacterium-mediated genetic transformation of Sedum erythrostichum was studied by introducing a herbicide-resistant gene (phosphinothricin-N-acetyl-transferase) and a reporter gene (-glucuronidase, GUS). Following co-cultivation with Agrobacterium on MS medium supplemented with 0.5mg/l -naphthaleneacetic acid (NAA) and 2mg/l 6-benzylaminopurine (BA) for 3days, leaf segments were transferred onto medium containing 300mg/l cefotaxime. When adventitious shoots developed directly near the margins of explants after 3weeks, they were transferred to selection medium with 25mg/l kanamycin. Of a total of 640 infected leaf explants, 24 (3.75%) produced kanamycin-resistant adventitious shoots; of these, 2.5% were GUS-positive. Transgenic plantlets were confirmed using polymerase chain reaction, Southern, and Northern analyses. Ninety-four percent of the transgenic plantlets were successfully transferred to soil and produced flowers. All GUS-positive transgenic plants were strongly resistant to Basta (phosphinothricin at 200mg/l) after spraying.