The establishment of occupational injury insurance accident prevention mechanism based on road transportation enterprises occupational injury insurance classification evaluation. With the help of system risk analysis, the paper carried out analysis on occupational injury risk influencing factors, built occupational injury risk evaluation index system which owned three grade indicators from the possibility and sequence severity of the accident. On this basis, it carried out occupational injury risk classification evaluation on road transportation enterprises, based on the situation of evaluation index impact on the enterprises with the help of fuzzy comprehensive evaluation. At last, the paper used an example to verify the proposed method.