The current diagnosis of stroke remains hampered and delayed due to lack of a suitable mechanism for the rapid, accurate, and analytically sensitive biomarker-based testing methods. In the present study, a MEKC method coupled with highly sensitive laser-induced fluorescence detection and precolumn derivatization was originally described for the rapid, sensitive determination of five amine biomarkers including homocysteine (Hcy), glutamic acid (Glu), putrescine (Put), spermine (Spm) and spermidine (Spd) of stroke in plasma samples from stroke patients. 3-(2-Furoyl)quinoline-2-carboxaldehyde (FQ) was selected as the fluorescence reagent and optimized for the advantageous high-throughput derivatization with total 30 μL reaction system in 384-well microplates for the rapid determination of the targeted analytes. Results showed that the proposed derivatization reaction approach coupled with MEKC-LIF was an efficient method for the rapid determination of five amine biomarkers of stroke in plasma samples.
