In this contribution we present our current research on polarization enhanced nuclear quadrupole resonance (NQR) detection at low frequencies with the emphasis on 14N NQR trinitrotoluene (TNT) detection at room temperature. Combination of proton—nitrogen level crossing polarization transfer and pulsed spin-locking sequence makes 14N NQR in TNT fast and sensitive enough to be used in routine detection of explosives. Enhancement factors for 14N NQR lines in TNT were calculated and compared with experimental values. Good agreement between measured and calculated signal enhancement factors was observed. 14N NQR signals in a 15 g trinitrotoluene sample of predominantly monoclinic modification were measured in 15 s in different polarization magnetic fields. The conditions for optimal polarization enhancement were determined.