In this paper, we adapt recently developed simulation-based sequential algorithms to the problem concerning the Bayesian analysis of discretely observed diffusion processes. The estimation framework involves the introduction of m−1 latent data points between every pair of observations. Sequential MCMC methods are then used to sample the posterior distribution of the latent data and the model parameters on-line. The method is applied to the estimation of parameters in a simple stochastic volatility model (SV) of the U.S. short-term interest rate. We also provide a simulation study to validate our method, using synthetic data generated by the SV model with parameters calibrated to match weekly observations of the U.S. short-term interest rate.