Background : A newly developed program, named cardioGRAF, enabled the evaluation of left ventricular (LV) systolic and diastolic temporal parameters for the estimation of heart failure using ECG-gated myocardial perfusion SPECT (GMPS). Objective : The feasibility of those global (g-) and regional (r-) parameters was validated to compare with gated equilibrium radionuclide angiography (ERNA) and speckle-tracking radial strain (STS) from echocardiography. Methods : Thirty-three patients were studied using GMPS and ERNA (n=11) or GMPS and STS (n=22). The following g- or r-parameters obtained by cardioGRAF and ERNA or STS were compared: time to en systole (TES), time from end systole to peak filling rate (TPF1), time from 0 to peak filling rate (TPF2), time to peak radial strain (TPS), time from peak strain to peak negative strain rate (TP-SR1), and time from 0 to peak negative strain rate (TP-SR2). Results : All g-parameters were successfully obtained by cardioGRAF and ERNA. The results demonstrated good correlations (g-TES: r=0.79, p<0.005; g-TPF1: r=0.75, p<0.02; TPF2: r=0.83, p<0.005). The differences were 11.9±31.8 ms in g-TES, 19.9±65.4 ms in g-TPF1, and 37.7±67.4 ms in g-TPF2. All r-parameters were successfully obtained by cardioGRAF. Eight patients and 12 segments were excluded because of the inadequate quality of routine echocardiography for STS analysis. However, r-parameters obtained by cardioGRAF were significantly correlated with those of STS (r-TES and r-TPS: r=0.61, p=1×10−8; r-TPF1 and r-TP-SR1: r=0.69, p=3×10−11; r-TPF2 and r-TP-SR2: r=0.76, p=2×10−15). The differences were 21.1±38.2 ms between r-TES and r-TPS, 7.0±123.4 ms between r-TPF1 and r-TP-SR1, and 38.1±111.5 ms between r-TPF2 and r-TP-SR2. Conclusion : The feasibility of evaluating systolic and diastolic temporal parameters by a new program was validated. This program has the potential to evaluate both diastolic and systolic heterogeneous wall motions which express dyssynchrony in heart failure.