A rod-coil amphiphilic organometallic oligomer based on o-ferrocenylcarbonyl benzoic acid (FcBA) as hydrophobic short rod and poly(ethylene glycol)17 (PEG17) as hydrophilic coil chain was synthesized via Friedel–Crafts acylation and esterification reaction and characterized by 1H-NMR and FT-IR analyses. The rod-coil oligomer FcBA-PEG17 with short-rod insoluble FcBA segment and long-coil PEG17 segment could self-assemble into multi-morphological aggregates in water such as sphere, rod, strip and vesicle at different initial concentrations, which were characterized by transmission electron microscopy. Interestingly, two different kinds of vesicles were observed, where disfigured vesicles formed at low initial concentration and ideal vesicles at high initial concentration. And the disfigured vesicles can be transferred into ideal vesicles at lower initial concentration through the threading of rigid α-CDs onto the coil PEG17 chains. A possible mechanism for the formation of multi-morphological self-assembled micelles was also proposed.