Recent developments have shown the potential of digital in-line holography for diagnostics in fluids. This new method provides a low-cost and easy access method for measuring both size and velocity of small particles in a volume. Here it is shown that by applying traditional image processing tools on the particle images digitally reconstructed, statistically reliable results on particles size and location are provided. The method is experimentally illustrated by glass microspheres that are moving in a turbulent flow generated by an annular jet. A comparison with the histogram diameters provided by a common diffraction particle sizer are presented.