Quaternary ammonium salts (otherwise known as “quats”) commonly form the foundation of formulations in the antimicrobial industry. Many studies have been conducted on the biological activity of surfactants derived from fatty acids viz. lauric acid, myristic acid, stearic acid and palmitic acid and palm fatty acid with polyamine, i.e. 1(2-hydroxyethylpiperazine). The present paper investigates the synthesis and surface-active properties of esteramide quats derived from the esterification of the above-mentioned fatty acids and 1(2-hydroxyethylpiperazine) followed by quaternization using dimethyl sulphate (DMS). These derivatives were fully characterized by using Fourier transform infrared spectroscopy, 1H nuclear magnetic resonance (NMR) spectroscopy, and 13C-NMR spectroscopy. In addition to the cationic content, surface tension, CMC (critical micelle concentration), fabric softening, rewettability, emulsification and dispersing power were determined as their surface-active properties.