In present study, yeast strains YGM1 and YGM2 were evaluated and the selected strain YGM1 was evaluated for its ethanol producing efficiency using varying substrate combinations. Both strain YGM1 and YGM2 were able to grow in wide range of temperature from 25 to 40 °C. Based on cfu ml−1 and corresponding absorbance values of two, YGM1 was rapid multiplier and better performer at 37 °C. During fermentation, pH of the substrate first decreased and then increased. Alcohol producing ability of YGM1 in substrates; 100 % pomace, 75 % pomace, 25 % molasses, 25 % pomace: 75 % molasses and 100 % molasses was compared. Alcohol producing ability is directly related to sugar utilization maximum amount of ethanol, 5.02 % was produced in pomace: molasses (75 %:25 %) and minimum 2.33 % in 100 % molasses. Maximum ethanol production, 5.02 % corresponded to maximum sugar utilization (11.31 %) in the 75 % pomace: 25 % molasses. Thus out of four combinations, 75 % pomace: 25 % molasses was better substrate for alcohol production.