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The problems of enzyme production are examined in the light of molecular biological events. an understanding of the dynamics of protein synthesis that occur during transient growth are reviewed to provide insight into the potentials and the limitations of differential enzyme synthesis.
After an introduction by a chapter on enzyme denaturation by physical chemical, and biological agents, methods for the stabilization of water-soluble enzymes are discussed in this review. this includes screening for intrinsically stabilized enzymes, addition of stabilizing agents such as substrates, solvents, salts or polymers, and chemical modification (e.g. acylation, reticulation, glycosidation...
Coenzymes are classified according to their needs for regeneration in coenzyme requiring enzymatic reactions. The activities of various forms of immobilized NAD(H) are compared. different coenzyme regeneration methods are discussed and their merits assessed. Reachtors that are suitable for coenzyme requiring enzymatic conversion are presented.
An integral study of chemical reaction engineering and process development including economic aspects was carried out using the optical resolution of aminoacids by means of native and carrier-fixed acylase as example. Continuous operation is not only possible with carrier-fixed enzymes but also with native enzymes using ultrafiltration devices for catalyst retention. It could experimentally be proved...
A number of simple models of affinity chromatography are presented which can be used individually or in combination to predict such results as 1) when a column will become saturated with enzyme, 2) when a peak will emerge from a column and how sharp it will be, 3) how much purification one can expect under a given set of conditions, and 4) what concentration of a competitive inhibitor is needed to...
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