Bacterial vaginosis can be treated by restoring the normal vaginal flora using lactobacilli.Lactobacillus crispatus KLB46 that was isolated from the human vagina has a strong antimicrobial activity and was grown in a batch and in a continuous fermentor. During batch cultivation, the maximum specific growth rate ofL. crispatus KLB 46 was 0.63 h−1 and the highest viable cell count (1.9×109 CFU/mL) was obtained at pH 5.5.L. crispatus KLB 46 did not grow well at either pH 3.5 or 7.5. During continuous cultivation, the highest viable cell count (1.53×109 CFU/mL) was obtained at a dilution rate of 0.32 h−1. However, the maximum productivity of viable cells was obtained at a dilution rate of 0.52 h−1, and was 7.33×1011 CFU L−1 h−1, that is approximately 5 times higher than that obtained from batch culture.