Lipids of corn, wheat, and triticale distiller’s dried grains with solubles (DDGS) were extracted with supercritical carbon dioxide (SC-CO2) and converted to biodiesel (fatty acid methyl esters; FAME) in a continuous SC-CO2 bioreactor using immobilized lipase. Different properties of the products obtained were characterized. FAME content of the products ranged between 87.1 % (corn DDGS lipid biodiesel; CB) and 92.0 % (triticale DDGS lipid biodiesel; TB). CB had a higher viscosity (6.24 mm2/s) than those of wheat DDGS lipid biodiesel (WB) (5.97 mm2/s) and TB (5.67 mm2/s). Cloud point of CB, WB, and TB were −8.48, −6.52, and −5.04 °C, respectively. Oxidative stability of WB and TB was higher than that of CB. Heat of combustion of products was 39.4–39.6 kJ/g. Continuous bioconversion of DDGS lipids to biodiesel in SC-CO2 is a simple, efficient, and “green” alternative to the conventional process and targets new opportunities for the biodiesel industry.