The effect of immobilized hyaluronidase on stem and progenitor cells of the lungs was studied on the model of partially reversible toxic bleomycin-induced pulmonary fibrosis in C57Bl/6 mice. During the inflammation phase, immobilized hyaluronidase reduced infiltration of alveolar interstitium with hemopoietic stem cells Sca-1+, c-Kit+, CD34–, (CD3, CD45R (B220), Ly6C, Ly6G (Gr1), CD11b (Mac1), TER-119)–. Improvement of histological parameters of bleomycin lungs during the phase of collagen fiber deposition after the treatment was accompanied by accumulation of mesenchymal multipotent stromal cells (CD31–, CD34–, CD45–, CD44+, CD73+, CD90+, CD106+decrease in the population of pan-hemopoietic cells (CD45+), accelerated restoration of the content of endothelial cells, and inhibition of clonal activity of fibroblast precursors (CD45–).