The purpose of the present study was to investigate student gender, subject area, and grade level differences in students' perceptions of their classroom learning environment. Over 13,000 students from 96 urban elementary, middle, and high schools that served predominantly minority students completed a modified version of the Classroom Environment Scale (CES) and the Instructional Learning Environment Questionnaire (ILEQ). Female students generally reported higher scores for their perceptions of the learning environment than did male students. There were very few differences by subject area, but there were many statistically and educationally significant differences by grade level. In general, middle school classes had less favorable perceptions of their learning environment than did either elementary or high school classes.