The chiral MOF InH(d-C 10 H 14 O 4 ) 2 has a left-handed helical structure and adopts a diamond network. In this work, three capillary columns (A, B, and C) containing InH(d-C 10 H 14 O 4 ) 2 , peramylated β-cyclodextrin and sodium chloride, and InH(d-C 10 H 14 O 4 ) 2 and peramylated β-cyclodextrin have been prepared and their respective enantioseparation abilities have been investigated. The polarity of the MOF is moderate, whereas peramylated β-cyclodextrin provides a stationary phase of low polarity. The numbers of theoretical plates (plate m −1 ) of the three columns have been measured with n -dodecane at 120 °C, which followed an increasing order of A (2,973) < B (3,563) < C (3,665). 1-Phenylethanol, dihydrocarvyl acetate, citronellal, leucine, methyl l-β-hydroxyisobutyrate, limonene, and menthol were separated with different resolutions on columns A, B, and C. The relative standard deviations (RSDs) in the run-to-run and column-to-column retention times were 0.50 and 2.50 %, respectively. The test results indicated that the incorporated InH(d-C 10 H 14 O 4 ) 2 enhanced the separations of racemates on a peramylated β-cyclodextrin stationary phase, resulting in high column efficiency and good reproducibility in gas chromatography.