To evaluate the quality of frozen surimi, the ATPase assay system was applied to pH stat. Surimi homogenate was prepared without washing procedures as a material for the measurement. Among the possible compounds frozen surimi contains, Mg ion slightly inhibited Ca-ATPase activity, while sugars or polyphosphate did not. Thus, the surimi homogenate was directly used for ATPase assay. The quality of surimi samples with various commercial grades were analyzed by measuring Ca-ATPase activity and protein content to obtain ATPase total activity. SA grade surimi showed high total activity, and was clearly distinguished from the second grade ones by the total Ca-ATPase activity. The specific Ca-ATPase activity contributed to the total activity more than the protein content. These results demonstrated that employment of surimi homogenate and the pH stat method made the total activity measurement of surimi easy and rapid.