Two kinds of new biocidal siloxane copolymers were synthesized, poly-[3(t-butylamino)propyl]methylsiloxane-co-(3-chloropropyl)methylsiloxane] and poly[dimethylsiloxane-co-(3-mercaptopropyl)methylsiloxane]-g-poly-2(t-butylamino)ethyl methacrylate]. Water-soluble derivatives of these copolymers, having charged ammonium groups, were also obtained. The copolymers were tested against two Gram-positive and three Gram-negative bacterial strains. The antibacterial tests for the water-soluble copolymers with charged ammonium groups were performed in aqueous solutions. Both these copolymers were active against the Gram-positive bacterial strains and inactive or moderately active against Gram-negative bacteria. The uncharged, insoluble, t-butylamine substituted copolymers were tested using a two-phase water-copolymer system. In this test both copolymers having the uncharged t-butylamine functions showed a high biocidal potency against the bacteria studied including those Gram-negative ones.