In the present work the uranyl hexacyanoferrate (K2UO2[Fe(CN)6]) is deposited on the palladized aluminum (Pd-Al) electrode from a $$ {\text{UO}}_{2}^{2 + } + {\text{Fe}}\left( {\text{CN}} \right)_{6}^{ - 3} $$ solution. Then the anodic stripping chronopotentiometry (ASCP) was used to strip the K2UO2[Fe(CN)6] from the Pd-Al surface. The operational conditions including: pH, K3Fe(CN)6 concentration, deposition potential, deposition time and stripping current were optimized. The ASCP calibration graph was linear in concentration range 10–460 μM. of $$ {\text{UO}}_{2}^{2 + } $$ and the detection limit was 8.5 μM. The interference of some concomitant ions during the deposition process of K2UO2[Fe(CN)6] was studied. The proposed method was successfully applied for analysis of some uranium mineral ores.