Hexagonal mesoporous silica (HMS) was prepared at ambient temperature by using dodecylamine (DDA) as the surfactant and tetraethyl orthosilicate (TEOS) as the silica precursor. The HMS materials were characterized by X-ray powder diffraction, N2 adsorption/desorption, scanning electron microscopy and transmission electron microscopy. The samples prepared at various addition rates of TEOS and various alcohol/water (R/H) ratios were investigated. As the addition rate of TEOS decreased, the size of HMS particles increased and the textural porosity decreased. Non-alcoholic solution was required to prepare non-spherical particles. The HMS with noodle-like structures was prepared in the absence of alcoholic solvent. These noodle-like particles were about 0.1μm in diameter and about submicrometer to several micrometers in length.