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Antibody phage display libraries have frequently been used for the isolation of antibody fragments against potential target structures on the cell surface of different types of cancer. Independent from the source of V-regions and the antibody format, two general strategies have been followed in generating antibody phage display libraries. Either non-immune so-called naïve (or synthetic) libraries...
The quality of antibodies selected from antibody gene libraries, in particular their affinity and the number of different clones per antigen, is a direct function of library diversity. Here, the optimization of every single step during library construction is of utmost importance for the quality. This chaper describes the generation of human naive or immune scFv antibody gene libraries by a two step...
Antibody fragments have gained strong attention in the last years and presumably represent one of the most promising molecules to construct new types of therapeutic proteins. Contrary to complete IgG, antibody fragments like scFv are not very stable and are frequently expressed at a very low level. This has prompted the development of new phage-displayed antibody libraries based on optimized scFv...
Libraries constructed from immunised non human primates (NHP; macaques here) are an appealing alternative to human libraries when immunization of Humans is difficult, as is so often the case. These phage-displayed libraries allow to obtain antibody fragments of very high affinities (nano- to picomolar), and diverse epitopes are targeted so that antibodies with the desired activity can be isolated...
Rabbits provide an interesting alternative to antibodies derived from the murine system. Furthermore, rabbits show an exceptional feature with respect to the mechanisms that are used for the generation of antibody diversity, i.e. a single VH gene that is preferentially used, and the diversity of the antibody repertoire being mainly generated by gene conversion and hypermutation, which facilitates...
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