A light-yellow coloured, Gram-stain negative, rod-shaped, aerobic, non-motile bacterium, designated THG-DN6.8 T , was isolated from a lotus pond near Donghaksa temple in Daejeon, South Korea. The strain was found to grow well on nutrient agar, optimally at pH 6.0–7.5, at temperature 25–28 °C and in the presence of 0.5 % NaCl. Based on 16S rRNA gene sequence analysis, strain THG-DN6.8 T was found to share the highest sequence similarity with Pedobacter koreensis KCTC 12536 T , followed by Pedobacter glacialis CCTCC AB 2012941 T , Pedobacter kyungheensis KACC 16221 T , Pedobacter caeni LMG 22862 T , Pedobacter insulae KCTC 12820 T and Pedobacter boryungensis KCTC 23344 T . The DNA G+C content was determined to be 36.8 mol%. In DNA–DNA hybridization tests, the DNA relatedness between strain THG-DN6.8 T and its closest phylogenetic neighbour P. koreensis was found to be below 10 %. The predominant isoprenoid quinone was identified as menaquinone MK-7 and the major polar lipid as phosphatidylethanolamine. The major fatty acids of strain THG-DN6.8 T were identified as iso-C 15:0 , C 16:0 , C 18:0 and C 16:1 ω 6 c and/or C 16:1 ω 7 c (summed feature 3). On the basis of the phenotypic characteristics, genotypic analysis and chemotaxonomic characteristics, strain THG-DN6.8 T is considered to represent a novel species of the genus Pedobacter , for which the name Pedobacter lotistagni sp. nov. is proposed. The type strain is THG-DN6.8 T (= KCTC 42229 T = JCM 30354 T ).