We investigated the influence of extracellular calcium concentration, caused by the dissolution of a bioactive glass with 60% of silicon (BG60S), on intracellular calcium (Cai 2 +) signals and expression of inositol 1, 4, 5-triphosphate receptors (InsP3R) in primary culture of osteoblasts. We found that BG60S caused an increase in Cai 2 + signals in this cell type. Additionally, osteoblasts pre-incubated in the presence of BG60S showed an increase in Cai 2 + when cells were stimulated with vasopressin. On the other hand, a decrease in Cai 2 + signals were observed in osteoblasts pre-treated with BG60S and stimulated with KCl. We furher found that in osteoblasts, the type I InsP3R is preferentially distributed in the nucleus while the type II InsP3R in the cytoplasm. Preincubation of osteoblasts with BG60S altered the receptor expression level, increasing the type I InsP3R in the nucleus and decreasing type II InsP3R in the cytosol. Together, our results showed that in osteoblasts, BG60S increased Cai 2 +signals and altered Cai 2 + machinery.