Abstract. In this study, we use the random principle to analyse the distributions of amino acids and amino acid pairs in human tumour necrosis factor precursor (TNF-) and its eight mutations, to compare the measured distribution probability with the theoretical distribution probability and to rank the measured distribution probability against the theoretical distribution probability. In this way, we can suggest that distributions with a high random rank should not be deliberately evolved and conserved and those with a low random rank should be deliberately evolved and conserved in human TNF-. An increased distribution probability in a mutation means probabilistically that the mutation is more likely to occur spontaneously, whereas a decreased distribution probability in a mutation means probabilistically that the mutation is less likely to occur spontaneously and perhaps is more related to a certain cause. The results, for example, show that the distributions of 30% of the amino acids are identical with their probabilistic simplest distributions, and the distributions of some of the remaining amino acids are very close to their probabilistic simplest distributions. With respect to probabilities of distributions of amino acids in mutations, the results show that mutations lead to an increase in eight probabilities, which are thus more likely to occur. Eight probabilities decrease and are thus less likely to occur. With respect to the random ranks against the theoretical probabilities of distributions of amino acids, the results show that mutations lead to an increase in seven and a decrease in seven probabilities, with two probabilities unchanged.