A recently developed transformation system for the pathogenic yeast Candida parapsilosis opened a venue for studying the biological phenomena of this species at the molecular level. However, the standard chemical method yielded only about 1103transformants/g of DNA, which is insufficient for certain types of experiment. With the aim of increasing the transformation efficiency, we employed two alternative methods for the introduction of plasmids into the recipient cells. Whereas biolistics resulted in about 5102transformants/g of plasmid DNA, electroporation was an order of magnitude more efficient than the chemical method. Pretreatment of cells with 100mM lithium acetate or 10mM dithiothreitol resulted in a 5-fold (5104) or a 10-fold (1105) increase in transformation efficiency, respectively. This high-efficiency transformation method should be suitable for experiments such as the screening of DNA libraries.