.Objective and design:
The effect of oxidized frying oils on PPARγ which is a potent inhibitor of inflammatory responses in the intestine is currently unknown. Thus, the present study aimed to explore the effect of oxidized frying oil on PPARγ DNA-binding and proinflammatory responses in intestinal epithelial cells.
Material and methods:
18 male pigs were fed two different diets containing either fresh fat or oxidized fat (n = 9 each). After 28 d, intestinal epithelial cells were isolated and analyzed for PPARγ DNA-binding, NF-κB DNA-binding and NF-κB target gene expression. In addition, markers of lipid peroxidation and antioxidant status were determined.
Results:
Feeding the oxidized fat slightly, but not significantly, activated PPARγ DNA-binding in intestinal epithelial cells when compared to fresh fat. In addition, oxidized fat increased the concentration of TBARS and reduced the concentrations of α-tocopherol and activities of antioxidant enzymes relative to fresh fat (P < 0.05). No effect of the oxidized fat was observed on NF-κB DNA-binding and NF-κB target gene expression in intestinal epithelial cells.
Conclusions:
The present study suggests that moderate PPARγ activation and induction of oxidative stress by oxidized frying oil have no implication for NF-κB-mediated proinflammatory gene expression in porcine intestinal epithelial cells.