A brassinosteroid inhibitor (Brz2001) was used to block the growth of roots, hypocotyls, and epicotyls of soybean seedlings, producing a dwarf phenotype. The application of 24-epibrassinolide completely reversed the inhibitory effects of Brz2001. Two other growth-promoting brassinosteroid analogs, MH5 and BB6, partially overcame the Brz2001-induced growth defects. The growth inhibition of Brz2001-treated seedlings was more effectively reversed by MH5 than by BB6, which may be due to the structural differences between the two compounds. These results indicate that the studied analogs may show brassinosteroid-like activity and therefore may have some practical use instead of brassinolide or its analogues.