Tetraploid cotton (Gossypium hirsutum) is cultivated in different regions of Iran and is considered as one of the most important economic crops of the country. Hybridization that brings about increased genetic diversity remains an important means to develop new elite cultivars in this crop. The present study considers cytogenetic study of some cotton cultivars and their F2 progenies obtained by different hybridization combinations. All cotton genotypes studied showed 2n = 4x = 52 chromosome number. They formed mostly bivalents in metaphase-I, although some amount of univalents and quadrivalents were also formed. The F2 plants differed significantly from their parental plants in their chiasma frequency and chromosome pairing. Meiotic abnormalities; laggard chromosome formation, stickiness and micronuclei formation were observed almost in all genotypes studied. Clustering grouped the F2 plants and parental genotypes in separate clusters indicating their cytogenetic distinctiveness. Cytogenetic differences obtained in F2 progenies indicate genetic variations present in the cotton cultivars of Iran which may be used in further hybridization and breeding programs to obtain elite cotton cultivars.