This chapter describes some examples of heparin-conjugated nanointerfaces for biomedical applications in terms of both development and application. First, heparin-conjugated linear and star shaped poly(lactic acid) (PLA-Hep and sPLA-Hep) to improve blood compatibility and its related biocompatibility of degradable polymers were introduced. Second, a heparin-conjugated PM system that consists of Tetronic and poly(ɛ-caprolactone) (PCL) is described as an injectable vehicle for an effective long-term delivery of growth factors. Finally, the heparin-immobilized small intestinal submucosa (SIS) was investigated to observe the effect of heparin immobilization on blood compatibility, in vitro fibroblast attachment and in vivo calcification of SIS. All of these examples demonstrate that heparin-conjugated nanointerfaces might be a versatile and promising tool to achieve advanced biomaterials.