Heading
Abstract
Aims/hypothesis. Hyperhomocysteinaemia increases cardiovascular risk in Type II (non-insulin-dependent) diabetes mellitus by augmenting oxidative stress and reducing nitric oxide availability. In vitro, nitric oxide decreases homocysteine by its conversion to the vasodilative and antioxidant compound S-nitrosohomocysteine. We investigated whether or not changes in nitric oxide availability decrease homocysteine concentrations in vivo.
Methods. The study group consisted of 20 normotensive, normolipidaemic, non-atherosclerotic Type II diabetic patients in good metabolic control (16 men, 51.2±1.4 years) and 15 healthy subjects (12 men, 48.1±1.5 years). Circulating concentrations of homocysteine, nitrite+nitrate and sulphydryl groups, a marker of oxidative stress, were assessed at baseline and then 5′, 10′, 30′ and 60′ after the intravenous infusion of either L-arginine (3 g in 10 ml saline), the nitric oxide precursor, or vehicle according to a double-blind cross-over randomized protocol.
Results. At baseline diabetic patients showed lower plasma sulphydryl group concentrations (491.8±16.9 vs 551.3±21.0 µmol/l, p<0.04) and nitrite+nitrate (21.4±0.8 vs 29.5±0.9 µmol/l, p<0.0001) and higher total homocysteine concentrations (11.1±0.5 vs 8.3±0.6 µmol/l, p<0.002) than the control subjects. After L-arginine infusion, blood pressure levels and total homocysteine concentrations (p≤0.05) decreased (peak at 5′ and 30′, respectively) whereas nitric oxide and sulphydryl group concentrations (p≤0.003) increased (peak at 10′ and 30′, respectively) in the patients and control subjects.
Conclusion/interpretation. Acute L-arginine infusion in both Type II diabetic patients and healthy subjects decreases plasma total homocysteine concentrations, counteract oxidative stress and increases the availability of nitric oxide.