This article examines the relationship between regulation and self-regulation in network governance. The comparative analysis is based on quantitative data collected in regional development networks in the Nordic countries. The theoretical debate, in which this analysis is embedded, focuses on the paradox in the way the relationship between regulation and self-regulation is described in network governance approach; the networks are seen to be self-organising, and yet the steering role of governmental bodies is emphasised. The results indicate that regulation and self-regulation are more than counterforces: they can coexist and affect the operations of the networks simultaneously.