Often vessels have a major effect on causing the paper properties of Eucalyptus [1–3]. They are picked from the paper during printing, causing a non-uniform paper surface, which affects the distribution of ink [1]. Vessel morphology and frequency are important selection indices in the quality breeding program for pulp production. However, the most appropriate core-sampling position has not as yet been reported for vessel morphology and frequency on a whole-tree basis. We examined and the representative height in the trunk to indicate whole-tree values of vessel morphology (length, radial, tangential and average diameters, and wall thickness) and frequency through the within-tree variation in Eucalyptus camaldulensis and E. globulus trees [4].