By using titanate nanotubes and HAuCl4 as precursor, gold-loaded TiO2 nanofibers (Au/TiO2NFs) are obtained after deposition-photodecomposition process, and the tubular supports are broken after the fabrication process. Catalytic performance of the obtained Au/TiO2NFs is evaluated for low-temperature CO oxidation and photodegradation of methyl orange under UV illumination, and the relationship between the catalytic activity and calcination treatment is investigated. After the Au/TiO2NFs are calcined, their catalytic activity for CO oxidation is enhanced, while the photocatalytic activity decreases.