The formation of nano-emulsions has been studied in water/ mixed nonionic surfactant/oil systems using two emulsification methods. In one method, the composition was kept constant and the temperature was changed (phase-inversion temperature, PIT, method), while in the other method, water was added dropwise to a solution of the mixed surfactants in oil at constant temperature (method B). The droplet size and stability were determined as a function of surfactant mixing ratio, W1, at 25 °C. The droplet size of nano-emulsions obtained by the PIT method is practically independent of W1 and falls in the range 60-80 nm. In contrast, the droplet size of nano-emulsions prepared by method B, is highly dependent on W1 and varies between 60 and 300 nm. At W1 values where the PIT or the hydrophile—lipophile balance temperature (Thlb) of the system is close to 25 °C, the droplet sizes of the nano-emulsions are similar for both emulsification methods. There are three equilibrium phases of the latter compositions: an aqueous micellar solution or oil-in-water microemulsion (W m), a lamellar liquid-crystalline phase and an oil phase (O) in addition, these nano-emulsions showed higher kinetic stability than those with lower W 1 values (higher T hlb) and consisting of two liquid phases (W m + O).