Two feeding experiments were conducted to elucidate growth performance of tiger puffer in a 10 m3 water volume closed system. In experiment 1, 1000 fish of 3.5 g average body weight were fed tiger puffer commercial feed twice daily to apparent satiation, 6 days a week for 224 days. Sand-filtered sea water was used and no water was exchanged during the rearing period. Immediately after cutting of lower teeth at day 112, daily feed consumption decreased greatly and 60 fish died in few days. Feeding rates recovered and then decreased gradually as nitrate levels increased from 600–1048 mg N/L. Fish grew to 343 g with 91% survival rate and 87% feed efficiency. Rearing conditions of experiment 2 were similar to experiment 1, except that culture water was exchanged to maintain the nitrate level less than 600 mg N/L during the 224-day experiment. Mortality and reduction of feed consumption occurred immediately after teeth cutting as was observed in experiment 1. Significant reduction of feed intake was not found during other rearing periods. Fish of 3 g grew to 303 g with 91% survival rate and 72% feed efficiency.