In the n-body problem a central configuration is formed if the position vector of each particle with respect to the center of mass is a common scalar multiple of its acceleration vector. We consider the problem: given a collinear configuration of four bodies, under what conditions is it possible to choose positive masses which make it central. We know it is always possible to choose three positive masses such that the given three positions with the masses form a central configuration. However for an arbitrary configuration of four bodies, it is not always possible to find positive masses forming a central configuration. In this paper, we establish an expression of four masses depending on the position x and the center of mass u, which gives a central configuration in the collinear four body problem. Specifically we show that there is a compact region in which no central configuration is possible for positive masses. Conversely, for any configuration in the complement of the compact region, it is always possible to choose positive masses to make the configuration central.