Objective
To investigate whether the cell adhesion molecule integrin αV could influence the radiosensitivity of human nasopharyngeal carcinoma CNE-2Z cells via effecting intercellular effect.
Methods
Human NPC cell line CNE-2Z was cultivated as three dimensional model using liquid overlay technique. Cell counting was employed to detect the radiosensitivity of CNE-2Z cells by blood cell counter before and after blocking integrin αV function; cell apoptosis was detected by flow cytometry using Annexin V/PI label.
Results
Three dimensional culture model of human NPC cell line CNE-2Z cells were successfully established. The integrin αV expression of CNE-2Z multicellular spheroids elevated as the dosage of radiotherapy increased. Blocking of integrin αV function leaded to higher radiosensitivity and more apoptotic cells of CNE-2Z multicellular spheroids.
Conclusion
Cell adhesion molecules integrin αV can influence the radiosensitivity of human nasopharyngeal carcinoma CNE-2Z cells and the inhibition of cell apoptosis might be its mechanism, proved by three dimensional culture model to mimic solid tumor in vivo.