The results of studying silicon concentrations and coexisting forms in surface water bodies of Ukraine with different hydrological regime, hydrophysical and hydrochemical characteristics are presented. Silicon distribution between its dissolved and suspended forms, as well as the relationship between the concentration of suspended silicon and the weight of suspended matter and its nature are discussed. The dominating form of silicon migration is shown to be its dissolved form (70.1–99.4% of its total content), while suspended form of silicon dominates in river waters with higher concentration of suspended matter of mineral origin, (56.1–81.5%). The results of studying silicon distribution between abiotic and biotic components of suspended matter, as well as between its dissolved forms with different charge signs are given. Dissolved silicon in the water bodies under study is shown to account for 93.0–99.0% of neutral fraction, where its compounds with molecular weight <0.2 kDa dominate.