Regenerative medicine aims to repair tissues or organs for restoring normal functions, which represents one of the greatest challenges in modern science and medicine. Diverse techniques and materials are required to truly understand the process of tissue repairing and build a proper scaffold for cells attachment, proliferation and differentiation. Functionalized nanomaterials with nanotechnologies are the ideal to solve most of the problems of regenerative medicine. Multifunctionalized nanoparticles and nanostructured biomaterials can be powerful tools for cell tracking and matrix-like scaffold rebuilding.