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An early embryonic stem cell line, EES-6 cells, was established fiom 2-cell stage embryos of ddY mice. The cells were maintained in an undifferentiated state with D-MEM/FlZ medium supplemented with 10% fetal bovine semm (FEW (GM) and lng of leukemia inhibitory factor (LIF) without any feeder cells. In this study, EES cells were cultured with a medium containing embryotrophic factors (ETFs) which promoted...
We outline the near 50-year history of leukemia-lymphoma (LL) cell lines — a key model system in biomedicine. Due to the detailed documentation of their oncogenomic and transcriptional alterations via recent advances in molecular medicine, LL cell lines may be fitted to parent tumors with a degree of precision unattainable in other cancers. We have surveyed the corpus of published LL cell lines and...
In the present study, we describe a new method of isolation and culture of human villous and extravillous trophoblasts from term placenta. The cultivation of trypsinized placental villous tissue explants, followed by the isolation of cells from outgrowth islets allows for obtaining a cytotrophoblast subpopulation that is free from contamination by other cell types. Compared to other methods, our protocol...
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