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Previously, we isolated the salt-sensitive mutant W2 by random Tn5 mutagenesis ofSinorhizobium meliloti 042BM strain, which contained single Tn5 insertion in the gene ofgreA, encoding transcription cleavage factor. To characterise the role of the novelgreA gene in salt tolerance ofS. meliloti 042BM strain, the full-length region ofgreA gene including putative promoter was cloned by PCR and thegreA...
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