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The method presented in here allows for the unequivocal differentiation between non-invasive samples of ocelots and margays, when used in combination with the protocol by Roques et al. (Mol Ecol Resour 11:171–175, 2011). It reduced costs and laboratory processing, decreasing the chances of contamination, and therefore facilitating the identification of large numbers of samples by simple PCR amplification,...
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