Journal of Biosciences

Items from 1 to 14 out of 14 results

article

Replica-moulded polydimethylsiloxane culture vessel lids attenuate osmotic drift in long-term cell cultures

Axel Blau, Tanja Neumann, Christiane Ziegler, Fabio Benfenati

Journal of Biosciences > 2009 > 34 > 1 > 59-69

An imbalance in medium osmolarity is a determinant that affects cell culture longevity. Even in humidified incubators, evaporation of water leads to a gradual increase in osmolarity over time. We present a simple replica-moulding strategy for producing self-sealing lids adaptable to standard, small-size cell-culture vessels. They are made of polydimethylsiloxane (PDMS), a flexible, transparent and...

article

Large cryptic internal sequence repeats in protein structures from Homo sapiens

R. Sarani, N. A. Udayaprakash, R. Subashini, P. Mridula, more

Journal of Biosciences > 2009 > 34 > 1 > 103-112

Amino acid sequences are known to constantly mutate and diverge unless there is a limiting condition that makes such a change deleterious. However, closer examination of the sequence and structure reveals that a few large, cryptic repeats are nevertheless sequentially conserved. This leads to the question of why only certain repeats are conserved at the sequence level. It would be interesting to find...

article

Human papillomavirus E6 and E7 oncoproteins as risk factors for tumorigenesis

Niladri Ganguly, Suraj P. Parihar

Journal of Biosciences > 2009 > 34 > 1 > 113-123

Human papillomavirus (HPV) is small, double-stranded DNA virus that infects mucosal and cutaneous epithelial tissue. HPV is sexually transmitted and the viral DNA replicates extrachromosomally. The virus is non-enveloped and has an icosahedral capsid. There are approximately 118 types of HPV, which are characterized as high-risk or lowrisk types. High-risk HPVs cause malignant transformation while...

article

Genetic analysis of foot-and-mouth disease virus serotype A of Indian origin and detection of positive selection and recombination in leader protease- and capsid-coding regions

S. B. Nagendrakumar, M. Madhanmohan, P. N. Rangarajan, V. A. Srinivasan

Journal of Biosciences > 2009 > 34 > 1 > 85-101

The leader protease (L^pro) and capsid-coding sequences (P1) constitute approximately 3 kb of the foot-and-mouth disease virus (FMDV). We studied the phylogenetic relationship of 46 FMDV serotype A isolates of Indian origin collected during the period 1968–2005 and also eight vaccine strains using the neighbour-joining tree and Bayesian tree methods. The viruses were categorized under three major groups...

article

Conserved water-mediated H-bonding dynamics of catalytic Asn 175 in plant thiol protease

Tapas K. Nandi, Hridoy R. Bairagya, Bishnu P. Mukhopadhyay, K. Sekar, more

Journal of Biosciences > 2009 > 34 > 1 > 27-34

The role of invariant water molecules in the activity of plant cysteine protease is ubiquitous in nature. On analysing the 11 different Protein DataBank (PDB) structures of plant thiol proteases, the two invariant water molecules W1 and W2 (W220 and W222 in the template 1PPN structure) were observed to form H-bonds with the O_b atom of Asn 175. Extensive energy minimization and molecular dynamics simulation...

article

Construction of an infectious cDNA clone of foot-and-mouth disease virus type O₁BFS 1860 and its use in the preparation of candidate vaccine

M. Hema, D. Chandran, S. B. Nagendrakumar, M. Madhanmohan, more

Journal of Biosciences > 2009 > 34 > 1 > 45-58

Foot-and-mouth disease virus (FMDV) serotype O is the most predominant among the endemic serotypes in India. A stable, full-length cDNA clone of FMDV type O₁BFS 1860 preceded by a bacteriophage T7 polymerase promoter was assembled in a plasmid vector pGEM^R-7Zf(−). An ∼8.2 kb PCR product was amplified from the cDNA clone and a full-length RNA was generated from it by in vitro transcription. Transfection...

article

Immunization with a synthetic robustoxin derivative lacking disulphide bridges protects against a potentially lethal challenge with funnel-web spider (Atrax robustus) venom

Alfio Comis, Margaret Tyler, Ewan Mylecharane, Ian Spence, more

Journal of Biosciences > 2009 > 34 > 1 > 35-44

The venom of male Atrax robustus spiders is potentially lethal to primates. These spiders have been responsible for a number of human deaths. Robustoxin is the lethal toxin in the venom. It is a highly cross-linked polypeptide that has 42 amino acid residues and four disulphide bridges. If these bridges are broken, the resulting polypeptide is nontoxic. Robustoxin was chemically synthesized with all...

article

Molecular evolution of the E8 promoter in tomato and some of its relative wild species

Lingxia Zhao, Liya Lu, Lida Zhang, Aoxue Wang, more

Journal of Biosciences > 2009 > 34 > 1 > 71-83

The E8 gene is related to ethylene biosynthesis in plants. To explore the effect of the expression pattern of the E8 gene on different E8 promoters, the molecular evolution of E8 promoters was investigated. A total of 16 E8 promoters were cloned from 16 accessions of seven tomato species, and were further analysed. The results from 19 E8 promoters including three previously cloned E8 promoters (X13437,...

article

Nitrilase-catalysed conversion of acrylonitrile by free and immobilized cells of Streptomyces sp.

V. K. Nigam, A. K. Khandelwal, R. K. Gothwal, M. K. Mohan, more

Journal of Biosciences > 2009 > 34 > 1 > 21-26

The biotransformation of acrylonitrile was investigated using thermophilic nitrilase produced from a new isolate Streptomyces sp. MTCC 7546 in both the free and immobilized state. Under optimal conditions, the enzyme converts nitriles to acids without the formation of amides. The whole cells of the isolate were immobilized in agar-agar and the beads so formed were evaluated for 25 cycles at 50°C....

article

Classical embryology to molecular biology: a personal view of amphibian embryonic development

Horst Grunz

Journal of Biosciences > 2009 > 34 > 1 > 5-16

article

What history tells us XVI. A third pillar for molecular biology: Molecular embryology

Michel Morange

Journal of Biosciences > 2009 > 34 > 1 > 17-20

article

Neurospora as a model fungus for studies in cytogenetics and sexual biology at Stanford

Namboori B. Raju

Journal of Biosciences > 2009 > 34 > 1 > 139-159

Dodge’s early work (1927–1940) on Neurospora genetics and sexual biology inspired Beadle and Tatum at Stanford to use N. crassa for their landmark discovery that genes specify enzymes. Neurospora has since become a model organism for numerous genetic, cytogenetic, biochemical, molecular and population biology studies. Neurospora is haploid in the vegetative phase with a transient diploid sexual phase...

article

Dissecting the genetics of cardiomyopathy in India: A tale of ten steps

Partha P. Majumder

Journal of Biosciences > 2009 > 34 > 1 > 1-3

article

Tropospheric ozone as a fungal elicitor

Paolo Zuccarini

Journal of Biosciences > 2009 > 34 > 1 > 125-138

Tropospheric ozone has been proven to trigger biochemical plant responses that are similar to the ones induced by an attack of fungal pathogens, i.e. it resembles fungal elicitors. This suggests that ozone can represent a valid tool for the study of stress responses and induction of resistance to pathogens. This review provides an overview of the implications of such a phenomenon for basic and applied...

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2009

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Keywords

FOOT-AND-MOUTH DISEASE VIRUS (2)
ACTIVATOR RNAS (1)
ALIPHATIC NITRILES (1)
ASCOSPORE (1)
ASCUS DEVELOPMENT (1)
BIOTRANSFORMATION (1)
CANCER (1)
CATTLE (1)
CENTRAL NERVOUS SYSTEM (1)
CHROMOSOME REARRANGEMENTS (1)
COCHLIOBOLUS HETEROSTROPHUS (1)
COMPLEX DISEASE (1)
CONIOCHAETA TETRASPORA (1)
CONSERVED WATER IN MOLECULAR RECOGNITION (1)
DELETION (1)
DNA AND RNA HYBRIDIZATION (1)
E6 (1)
E7 (1)
E8 PROMOTER (1)
ELICITOR (1)
EMBRYONIC INDUCTION (1)
EVOLVABILITY (1)
FULL-LENGTH INFECTIOUS CDNA CLONE (1)
GENE REGULATION (1)
GFP-TAGGED GENES (1)
GROWTH FACTORS (1)
GUINEA PIGS (1)
HISTONE H1-GFP (1)
HPV (1)
HUMAN GENOME (1)
HYPEROSMOLARITY (1)
IDENTICAL AND SIMILAR SEQUENCE REPEATS (1)
IMMOBILIZATION (1)
IN VITRO TRANSCRIBED RNA (1)
LONG-TERM CELL CULTURE (1)
MD SIMULATION (1)
MEIOTIC DRIVE (1)
MEIOTIC SILENCING (1)
MOLECULAR EVOLUTION (1)
MONKEYS (1)
MYBPC3 (1)
NEUROSPORA SEXUAL BIOLOGY (1)
NITRILASE (1)
ONCOPROTEINS (1)
OZONE (1)
P53 (1)
PATHOGENS (1)
PDMS LIDS (1)
PDZ (1)
PEPTIDE IMMUNOGEN (1)
PH STABILIZATION (1)
PHYLOGENY (1)
PICORNAVIRIDAE (1)
PLANT CYSTEINE PROTEASE (1)
POSITIVE SELECTION (1)
PRB (1)
PROPENSITY (1)
PROTECTION (1)
RECOMBINATION (1)
REPLICA MOULDING (1)
SPEMANN-MANGOLD-ORGANIZER (1)
SPIDER TOXIN (1)
SPORE KILLERS (1)
STREPTOMYCES (1)
STRESS (1)
STRUCTURAL PLASTICITY (1)
STRUCTURE-FUNCTION CORRELATION (1)
THERMOPHILIC (1)
THREE-DIMENSIONAL STRUCTURE (1)
TOMATO (1)
TRANSFECTION (1)
VACCINE (1)
VEGETALIZING FACTOR (ACTIVIN) (1)
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