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The efficiency of sheep is dependent on their health and well-being. The blood markers can be critical for improving of the physiological, nutritional and pathological status of sheep organism. The aim of this study was to test the hypotheses that the red and white blood cells and copper (Cu) and zinc (Zn) plasma contents are impacted by altitude and season. The ewes were kept at three farms. Blood...
Lignin is synthesized not only during morphogenesis of vascular plants but also in response to various stresses. Isolated Zinnia elegans mesophyll cells can differentiate into tracheary elements (TEs), and deposit lignin into cell walls in TE-inductive medium (D medium). Meanwhile isolated mesophyll cells cultured in hormone-free medium (Co medium) accumulate stress lignin-like substance during culture...
Vrana Lake is a coastal karst lake on Cres Island within the eastern Adriatic coast. It appears to have formed as a cryptodepression on karstified carbonate rocks, and is filled with fresh water. It is classified as an oligotrophic hydrosystem and serves as a natural water supply reservoir (max. depth 74.5 m; surface area 5.5 km2; water volume 220 × 106 m3). The aim of this study was to assess the...
The present paper discusses preliminary data on population characteristics and ecology of the meadow viper Vipera ursinii in the Romanian Danube Delta. Using line transects and the Distance software, the size of the studied population was estimated at 321 (95% confidence interval: 166–618) individuals on a 62 ha area. The sex-ratio of the population was close to 1:1 and juvenile specimens were well...
The density, distribution and population structure of Opusia indica were studied through transects method. Two transects were delimited in a mangrove area of Korangi creek (24°79′ N/67°20′ E). On each transect, three 0.25 m2 quadrats were sampled at three tidal levels on a monthly basis during low tide. A total of 1919 crabs were obtained, of which 775 were males, 945 were non-ovigerous females and...
The gene encoding the glycine oxidase from Bacillus subtilis strain R5 (goxR) was cloned and expressed in Escherichia coli. The gene consisted of 1,110 nucleotides that encoded a protein (GoxR) of 369 amino acid residues with a molecular mass of 40,761 Da. The GoxR exhibited 98.6% identity with glycine oxidase from B. subtilis strain 168. Gene expression and purification of the recombinant GoxR were...
Glucoamylase from the yeast Saccharomycopsis fibuligera R64 (GLL1) has successfully been purified and characterized. The molecular mass of the enzyme was 56,583 Da as determined by mass spectrometry. The purified enzyme demonstrated optimum activity in the pH range of 5.6–6.4 and at 50°C. The activity of the enzyme was inhibited by acarbose with the IC50 value of 5 μM. GLL1 shares high amino acid...
We describe a new braconid species of the genus Orgilus (O. radialiformis Beyarslan, sp. n.) and give data for 19 species of Microtypinae and Orgilinae from Turkey, of which 12 are new to the Turkish fauna. The new species is compared with the related species Orgilus (O.) radialis. Information on the distribution, host relationships of species and diagnostic characters are provided for each species.
The morphometric analyses and genetic variability assessed by RAPD markers have been used to analyse relations among six Serapias taxa from Croatia (S. istriaca, S. pulae originally described as hybrid, S. ionica, S. vomeracea, S. lingua and S. cordigera). S. istriaca distributed in southern Istria and the island of Lošinj and S. pulae stenoendemic taxon distributed only in southern Istria S. ionica...
Sponges are one of the oldest metazoan phyla that are, due to their highly conservative nature, often referred to as the living fossils of multicellular animals. As such, they are a very important model for evolutionary, developmental and functional studies of Metazoa. Tubulin polymerization promoting proteins (TPPPs) are defined by the presence of p25-alpha domain (Pfam05517). Their functional characteristics...
Leptospirosis constitutes a major health problem in tropical and subtropical countries and is caused by pathogenic Leptospira. Immuno-magnetic separation (IMS) is considered to be an effective pre-enrichment method to isolate Leptospira from liquid specimen. We applied an inexpensive and simple IMS protocol using zero-length cross-linkers to immobilize polyclonal anti-leptospiral antibodies onto magnetic...
Family Apocynaceae is an economically important family grown as ornamental plants and many wild species have medicinal uses as well. The aim of the present study was to understand the level and pattern of genetic variability among the selected individuals of Apocynaceae. For this purpose, three species of different genera of Apocynaceae, Thevetia peruviana, Alstonia scholaris and Catharanthus roseus...
The effectiveness of the predatory mite Typhlodromus pyri and of an application of Polysulphide-Ca was compared during 2006–2008. The experiment showed differences in infestation levels of pear leaves caused by Eriophyes pyri. The highest percentage of leaves with symptoms was found in untreated control group (⊘ 20.9%); the lowest percentage was found in plots treated with T. pyri (⊘ 3.7%); and a...
Post-glacial survival, potential migration routes, genetic diversity and phylogeography of the boreal moss species Rhytidium rugosum have been studied. This species is considered to be one of glacial relics of the wide but scattered Holarctic range. According to molecular data sampling from the selected European, American and Asian populations high genetic diversity of this species is present, even...
This study aimed to evaluate the influence of alterations in pluviosity and ecological variables on microphytoplankton (> 20 μm) structure (composition, richness, diversity, and abundance) and its biomass (chlorophyll-a), comparing different regions in a stretch of the low Iguaçu River and in some tributaries. Phytoplankton was sampled in 10 stations (5 in Iguaçu River and 5 in tributaries) during...
The hypothesis of the possible therapeutic potential of selected species of forage crops is discussed. Extracts from genotypes of Anthyllis sp., Astragalus sp., Coronilla sp., Lotus sp., Medicago sp., Melilotus sp., Onobrychis sp. and Trifolium sp. were prepared and tested for proteinase inhibition and antioxidant activities. We found that Trifolium pratense accession POLKIE99-3 expressed the highest...
In this study, morphological and anatomical features of Ornithogalum nutans and O. boucheanum, two relative and morphologically similar species growing in European Turkey, were investigated. These species showed some important anatomical differences with regard to leaf anatomy while they have identical features in stem. The stem anatomy of these two species displays the common properties of monocotyledons...
In this study we performed a phylogenetic analysis of a culturable bacterial community isolated from heavymetal-contaminated soil from southwest Slovakia using 16S rRNA (16S rDNA) and heavy-metal resistance genes. The soil sample contained high concentrations of nickel (2,109 mg/kg), cobalt (355 mg/kg) and zinc (177 mg/kg), smaller concentrations of iron (35.75 mg/kg) and copper (32.2 mg/kg), and...
Steroids both in seminal fluid (SF) and blood serum (BS) as well as the milt quality (sperm motility and sperm production) were investigated during final maturation of Persian sturgeon. The BS levels of testosterone (T), 11-Ketotestosterone (11-KT), progesterone (P), 17α,20β,21-trihydroxy-4-pregnen-3-one (20βS), cortisol (C) and 17α,hydroxyprogesterone (OHP) elevated after pituitary preparation (PP)...
The paper is aimed at evaluation of the influence of two different agricultural management forms on harvestman (Opiliones) communities structure in relation to the optimization of land use on the model territory of Agricultural Cooperative (AC) Očová (Central Slovakia). The research was carried out using pitfall trapping through growing seasons from 2005 to 2007. Harvestmen were captured at four pairs...
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