In Vitro Cellular & Developmental Biology - Animal

Items from 1 to 20 out of 49 results

article

Increased activity of the calcineurin-nuclear factor of activated T cells pathway in squirrel monkey B-lymphoblasts identified by powerblot^™

Katherine L. Gross, Eugene A. Cioffi, Jonathan G. Scammell

In Vitro Cellular & Developmental Biology - Animal > 2004 > 40 > 1-2 > 57-63

Summary New World primate-derived cell lines were instrumental in identifying the primary factors causing glucocorticoid resistance in these primate species. Their use is expanding because it has been recognized that some of these cell lines exhibit differential sensitivity to retroviral infection. To enhance their utility as cell models, we have further characterized one of these cell lines, squirrel...

article

Characterization of human melanoma cell lines according to their migratory properties in vitro

Lius G. Quiñones, Ivette Garcia-Castro

In Vitro Cellular & Developmental Biology - Animal > 2004 > 40 > 1-2 > 35-42

Summary The migratory responses of four human melanoma cell lines (A-2058, DEMEL, HTB-63, and HTB-72), using chemotaxis (CTX) and haptotaxis (HPTX) assays, were studied. The attractants were three extracellular matrix components (EMCs), fibronectin, laminin, and collagen type IV. The conditioned media (CM) of each cell line were used to study autocrine and paracrine responses. A screening and sensitive...

article

Action of major insecticide groups on insect cell lines of the beet armyworm, Spodoptera exigua, compared with larvicidal toxicity

Luc Decombel, Guy Smagghe, Luc Tirry

In Vitro Cellular & Developmental Biology - Animal > 2004 > 40 > 1-2 > 43-51

Summary In a first series of experiments, the biological response of a continuous cell line of the beet armyworm, Spodoptera exigua, was tested with different groups, of insecticides with different modes of actions; acetylcholinesterase inhibitors, acetycholine receptor agonists, inhibitors and uncouplers of oxidative phosphorylation, site I electron transport inhibitors, gamma-aminobutyric acid receptor...

article

Enhancement of lipid bodies during differentiation of skeletal myofibroblasts of rat's fetus in vitro

Renata O. Pereira, Tecia Maria U Carvalho, Helene S. Barbosa, Luis Cristovão Porto, more

In Vitro Cellular & Developmental Biology - Animal > 2004 > 40 > 1-2 > 1-3

Summary Fibroblasts in vitro can acquire myofibroblast phenotype by the development of several biochemical and morphological properties of smooth muscle cells, particularly the expresion of alpha-smooth muscle actin. These cells play a major role in inflammatory responses and in wound repair through their production of growth factors, cytokines, and other soluble mediators. Lipid bodies (LB) are lipid-rich...

article

Induction of osteoblast aggregation, detachment, and altered integrin expression by bear serum

Michael Overstreet, Timothy Floyd, Anna Polotsky, David S. Hungerford, more

In Vitro Cellular & Developmental Biology - Animal > 2004 > 40 > 1-2 > 4-7

Summary Animal models have long been used to elucidate the mechanisms responsible for osteoporosis in humans. The American black bear, an animal that does not experience extensive bone loss normally associated with long-term immobilization (when hibernating), may provide an insight into the nature of the pathogenesis of the disease. Circulating growth and differentiation factors present in the serum...

article

Maintenance of midgut epithelial cells from dendroctonus valens (coleoptera: Scolytidae) in vitro

L. Sánchez, J. L. Andrade, R. Cisneros, G. Zúñiga

In Vitro Cellular & Developmental Biology - Animal > 2004 > 40 > 1-2 > 8-13

Summary This article describes the culture of epithelial cells from anterior and posterior midgut regions of adult Dendroctonus valens. Culture conditions were established, and cell adherence was improved by means of a new technique that allowed the cells to grow between two glass coverslips. Cytoplasmic projections occur as anterior midgut cells grow to confluence; these projections were not observed...

article

Explant-cell culture of primary mammary tumors from MMTV-c-Myc transgenic mice

Xu Fang Pei, Marcia S. Noble, Maria Antonietta Davoli, Edward Rosfjord, more

In Vitro Cellular & Developmental Biology - Animal > 2004 > 40 > 1-2 > 14-21

Summary We have established an explant-cell culture system for mammary gland tumors from c-myc oncogene-expresing trangenic mice and potentially other transgenic strains. By coating culture dish surfaces with fetal bovine serum and using culture media supplemented with low serum and growth factors, the mammary tumor specimens could be maintained in culture for over 3 mo. Throughout the culture period,...

article

Growth, immortalization, and differentiation potential of normal adult human proximal tubule cells

David E. Orosz, Philip G. Woost, Robert J. Kolb, Margaret B. Finesilver, more

In Vitro Cellular & Developmental Biology - Animal > 2004 > 40 > 1-2 > 22-34

Summary Human proximal tubule epithelial cell lines are potentially useful models to elucidate the complex cellular and molecular details of water and electrolyte homeostasis in the kidney. Samples of normal adult human kidney tissue were obtained from surgical specimens, and S1 segments of proximal convoluted tubules were microdissected, placed on collagen-coated culture plate inserts, and cocultured...

article

Effects of excess and deprivation of serotonin on in vitro neuronal differentiation

Elena Menegola, Maria Luisa Broccia, Francesca Renzo, Valentina Massa, more

In Vitro Cellular & Developmental Biology - Animal > 2004 > 40 > 1-2 > 52-56

Summary The neutrotransmitter serotonin (5HT) possesses developmental functions in vertebrates and invertebrates. Rodent embryos express 5HT receptors even before neural development, but the role of this neurochemical seems to be particularly important during axonal morphogenesis and differentiation and in neural crest cell migration. Moreover, 5HT inhibitors are teratogenic in mammals, inducing brain...

article

Development and partial characterization of heliothine cell lines from embryonic and differentiated tissues

Cynthia L. Goodman, Amy A. Wang, Henda Nabli, Arthur H. Mcintosh, more

In Vitro Cellular & Developmental Biology - Animal > 2004 > 40 > 3-4 > 89-94

Summary The goal of this study was to generate cell lines from a variety of insect tissues that could be useful for developing in vitro assays with tissue-specific properties. In this article, we describe the establishment of new cell cultures from differentiated (primarily neural) and undifferentiated tissues (primarily embryonic) and their initial characterization. Cell lines were established from...

article

Effects of ethanol and hydrogen peroxide on mouse limb bud mesenchyme differentiation and cell death

Corey S. Johnson, Maria R. Blanton, E. Sidney Hunter

In Vitro Cellular & Developmental Biology - Animal > 2004 > 40 > 3-4 > 108-112

Summary Many of the morphological defects associated with embryonic alcohol exposure are a result of cell death. During limb development, ethanol administration produces cell death in the limb and digital defects, including postaxial ectrodactyly. Because an accumulation of reactive oxygen species (ROS) is produced in adult and embryonic tissues by ethanol exposure, this investigation examines the...

article

Identification of cells responsible for urokinase-type plasminogen activator synthesis and secretion in human diploid kidney cell cultures

Safedin Beqaj, Anuja M. Shah, Jon M. Ryan

In Vitro Cellular & Developmental Biology - Animal > 2004 > 40 > 3-4 > 102-107

Summary Human urokinase-type plasminogen activator (uPA) is a serine protease that converts plasminogen to plasmin. It is produced and secreted by a variety of different human cells in vivo and in vitro. We have studied human diploid kidney cell (HKC) cultures prepared from neonatal kidney tissue and cultures of purified populations of HKC to determine which cells synthesize and secrete uPA into the...

article

An in vitro screening assay for inhibitors of proinflammatory mediators in herbal extracts using human synoviocyte cultures

Carmelita G. Frondoza, Afshin Sohrabi, Anna Polotsky, Phong V. Phan, more

In Vitro Cellular & Developmental Biology - Animal > 2004 > 40 > 3-4 > 95-101

Summary Tumor necrosis factor-α (TNF-α), cyclooxygenase (COX)-2, and prostaglandin (PG)E-2 play a critical role in the pathophysiology of arthritis. Tumor necrosis factor-α mediates induction of other cytokines, COX-2, PGs, and metallo-proteinases, which leads to cartilage degradation. We developed an in vitro human synoviocyte assay system for screening inhibitors of proinflammatory mediators in...

article

Development of rainbow trout hepatoma cell lines: Effect of pro-IGF-I EA4-peptide on morphological changes and anchorage-independent growth

Maria J. Chen, P. Peter Chiou, Bih-Ying Yang, Hung Chieh Lo, more

In Vitro Cellular & Developmental Biology - Animal > 2004 > 40 > 3-4 > 118-128

Summary Our laboratory has shown previously that recombinant rainbow trout Ea4 (rtEa4)-peptide of pro-insulin-like growth factor-I (pro-IGF-I) exhibited antitumor activities against cancer cell lines derived from various human cancer tissues (Chen et al., 2002; Kuo and Chen, 2002). To confirm that rtEa4-peptide can exhibit the same spectrum of antitumor activities in fish tumor cells, we had developed...

article

Isolation and molecular characterization of a mouse renal microvascular endothelial cell line

Silvina Gazzaniga, Lorena González, Alberto Mantovani, Annunciata Vecchi, more

In Vitro Cellular & Developmental Biology - Animal > 2004 > 40 > 3-4 > 82-88

Summary Murine endothelial cells (ECs) have proven difficult to obtain and maintain in culture. Long-term maintenance of normal ECs remains a difficult task. In this article we report the establishment of the first cellular line of renal microvascular endothelium obtained from normal tissue. Cells were isolated, cloned, and maintained by serial passages for longer than 24 mo, using endothelial cell...

article

Suppressive effects of ascorbate derivatives on ultraviolet-B-induced injury in hacat human keratinocytes

Shin Yasuda, Mikiro Tada, Koji Yamada, Kyoya Takahata

In Vitro Cellular & Developmental Biology - Animal > 2004 > 40 > 3-4 > 71-73

Summary The aging of skin, including sunburning, is caused by ultraviolet (UV) irradiation. Here, we examined the inhibitory effect of ascorbic acid (AsA) and its derivatives AsA 2-phosphate (AA-2P) and AsA 2-glucoside (AA-2G) on UV-B-induced cytotoxicity in HaCaT keratinocytes. Results show that cell viability significantly decreased when exposed to UV-B at 0.1–0.4 J/cm² in a dose-dependent manner...

article

Production of mosquito densonucleosis viruses by Aedes albopictus C6/36 cells adapted to suspension culture in serum-free protein-free media

Erica Suchman, Jonathan Carlson

In Vitro Cellular & Developmental Biology - Animal > 2004 > 40 > 3-4 > 74-75

Summary Mosquito densonucleosis viruses (MDVs) have the potential for use as biocontrol agents. To facilitate densovirus production, the Aedes albopictus mosquito cell line C6/36 was adapted to two commercially available serum-free protein-free media (SFPFM), Sf-900 II and Drosophila-SFM. Cells adapted more slowly to growth in Sf-900 II medium, but once adapted, they grew more rapidly and appeared...

article

Culture condition difference for establishment of new embryonic stem cell lines from the C57BL/6 and BALB/c mouse strains

Hossein Baharvand, Klaus Ingo Matthaei

In Vitro Cellular & Developmental Biology - Animal > 2004 > 40 > 3-4 > 76-81

Summary Embryonic stem (ES) cells are pluripotent cells derived from the inner cell mass of blastocysts. These cells are appropriate for creation of animal models of human genetic diseases, the study of gene function in vivo and differentiation into specific types as potential therapeutic agents for several human diseases. We describe here, the production of new ES cell lines from blastocysts recovered...

article

The effect of the bisphosphonate alendronate on viability of canine osteosarcoma cells in vitro

James P. Farese, Jenna Ashton, Rowan Milner, Linda-Lee Ambrose, more

In Vitro Cellular & Developmental Biology - Animal > 2004 > 40 > 3-4 > 113-117

Summary The objective of this study was to determine the effect of alendronate on the viability of canine osteosarcoma cells and nonneoplastic canine cells. The sample population was composed of canine osteosarcoma tumor cells. Osteosarcoma cells and canine fibroblasts were maintained in culture under standard conditions. The MTT assay for cell viability was performed after 24, 48, and 72 h of incubation...

article

Upregulation and protein trafficking of aquaporin-2 attenuate cold-induced osmotic damage during cryopreservation

Wenjun Wang, Robert N. Ben

In Vitro Cellular & Developmental Biology - Animal > 2004 > 40 > 3-4 > 67-70

Summary Aquaporins (AQPs) are a recently discovered family of proteins that function as transmembrane water channels. These proteins regulate the delicate osmotic balance across the cell plasma membrane. Given that osmotic damage is the major contributing factor to cell death during freezing, we hypothesized that regulation of AQPs may have an unrealized role in protecting cells from osmotic damage...

Year:
2004

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40 (49)

Keywords

CELL ATTACHMENT (2)
CELL CULTURE (2)
DIFFERENTIATION (2)
EXTRACELLULAR MATRIX (2)
HUMAN (2)
IMMORTALIZATION (2)
MOUSE (2)
BACILLUS THURINGIENSIS (1)
ESCHERICHIA COLI (1)
FUSARIUM TOXINS (1)
HELICOVERPA ZEA (1)
HELIOTHIS VIRESCENS (1)
N-ACETYLGALACTOSAMINE (1)
TRICHOPLUSIA NI CELL LINE TNH5 (1)
5HT (1)
ADHESION (1)
AGGREGATION (1)
ALCOHOL (1)
ALENDRONATE (1)
ALKALINE SERINE PROTEASE (1)
ANCHORAGE-INDEPENDENT CELL GROWTH (1)
ANTITUMOR EFFECT (1)
APOPTOSIS (1)
AQUAPORIN (1)
ASCORBIC ACID (1)
ASCORBIC ACID 2-GLUCOSIDE (1)
ASCORBIC ACID 2-PHOSPHATE (1)
AUTOCRINE MOTILITY FACTORS (1)
AUTOTAXIN (1)
AVIAN (1)
BALB/C (1)
BARK BEETLES (1)
BEAR (1)
BIOCHEMICAL INDICATORS (1)
BISPHOSPHONATE (1)
BROMODEOXYURIDINE (1)
C57BL/6 (1)
CALCIUM (1)
CANINE (1)
CD31 (1)
CELL ADHESION (1)
CELL AGGREGATION (1)
CELL CLONING (1)
CELL CULTURE METHODS (1)
CELL CULTURES (1)
CELL CYCLE (1)
CELL DENSITY (1)
CELL DIFFERENTIATION (1)
CELL IDENTITY (1)
CELL INVASION (1)
CELL ISOLATION (1)
CELL LINE (1)
CELL POLARITY (1)
CHEMOTAXIS (1)
CHONDROGENESIS (1)
CILIUM (1)
CLARA (1)
COLLAGEN TYPE II (1)
COLONIC CRYPT (1)
CRUDE CLOSTRIDIUM HISTOLYTICUM COLLAGENASE (1)
CRYOPRESERVATION (1)
CRYSTAL VIOLET (1)
CULTIVATION (1)
CYTOKERATIN 19 (1)
CYTOKINES (1)
CYTOTOXICITY (1)
DENSOVIRUS (1)
DEOXYRIBONUCLEIC ACID ENDOREDUPLICATION (1)
ELECTROLYTE TRANSPORT (1)
ELECTROPHYSIOLOGY (1)
EMBRYO IMPLANTATION (1)
EMBRYONIC (1)
EMBRYONIC CELLS (1)
EMBRYONIC STEM CELLS (1)
ENDOTHELIAL CELLS (1)
ENDOTHELIUM (1)
ENTEROCYTES (1)
EPITHELIAL CULTURE (1)
EPITHELIUM (1)
EXPRESSION (1)
FBS (1)
FEEDER LAYER (1)
FEEDER SUPPORT (1)
FERTILITY CYCLE (1)
FIBROBLASTS (1)
FUSION (1)
GELATIN (1)
GENE EXPRESSION (1)
GENETIC ERRORS (1)
GINGER EXTRACT (1)
GLUTATHIONE (1)
GLYCOSYLATION (1)
GOBLET (1)
HCG (1)
HELA CONTAMINANTS (1)
HEMOLYMPH (1)
HEPATOCYTE-LIKE CELL LINES (1)
HIS TAG (1)
HL-60 (1)
ICAM (1)
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INFONA - science communication portal

In Vitro Cellular & Developmental Biology - Animal

Increased activity of the calcineurin-nuclear factor of activated T cells pathway in squirrel monkey B-lymphoblasts identified by powerblot^™

Characterization of human melanoma cell lines according to their migratory properties in vitro

Action of major insecticide groups on insect cell lines of the beet armyworm, Spodoptera exigua, compared with larvicidal toxicity

Enhancement of lipid bodies during differentiation of skeletal myofibroblasts of rat's fetus in vitro

Induction of osteoblast aggregation, detachment, and altered integrin expression by bear serum

Maintenance of midgut epithelial cells from dendroctonus valens (coleoptera: Scolytidae) in vitro

Explant-cell culture of primary mammary tumors from MMTV-c-Myc transgenic mice

Growth, immortalization, and differentiation potential of normal adult human proximal tubule cells

Effects of excess and deprivation of serotonin on in vitro neuronal differentiation

Development and partial characterization of heliothine cell lines from embryonic and differentiated tissues

Effects of ethanol and hydrogen peroxide on mouse limb bud mesenchyme differentiation and cell death

Identification of cells responsible for urokinase-type plasminogen activator synthesis and secretion in human diploid kidney cell cultures

An in vitro screening assay for inhibitors of proinflammatory mediators in herbal extracts using human synoviocyte cultures

Development of rainbow trout hepatoma cell lines: Effect of pro-IGF-I EA4-peptide on morphological changes and anchorage-independent growth

Isolation and molecular characterization of a mouse renal microvascular endothelial cell line

Suppressive effects of ascorbate derivatives on ultraviolet-B-induced injury in hacat human keratinocytes

Production of mosquito densonucleosis viruses by Aedes albopictus C6/36 cells adapted to suspension culture in serum-free protein-free media

Culture condition difference for establishment of new embryonic stem cell lines from the C57BL/6 and BALB/c mouse strains

The effect of the bisphosphonate alendronate on viability of canine osteosarcoma cells in vitro

Upregulation and protein trafficking of aquaporin-2 attenuate cold-induced osmotic damage during cryopreservation

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