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Ruppia cirrhosa, a temperate seagrass growing in brackish water, featured a high capacity for HCO3− utilisation, which could operate over a wide pH range (from 7.5 up to 9.5) with maintained efficiency. Tris buffer inhibited this means of HCO3− utilisation in a competitive manner, while addition of acetazolamide, an inhibitor of extracellular carbonic anhydrase activity, caused a 40–50% inhibition...
A kinetic analysis of the photosynthesis inhibition by buffers allowed quantification of some components of the carbon concentrating mechanism (CCM) of the brown macroalga Laminaria saccharina. The CCM was based on the presence of acid regions outside the plasma membrane that increased the CO2 concentration available for photosynthesis by 10–20 times above that of the bulk medium at alkaline pH. Furthermore,...
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