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Lettuce mosaic potyvirus (LMV) can be very destructive on lettuce crops worldwide. The LMV strain 0 (LMV-0) coat protein (CP) gene was engineered for expression in plants. It was introduced into three susceptible cultivars of Lactuca sativa using an improved procedure for transformation and regeneration of lettuce, by co-cultivation of leaf explants with Agrobacterium tumefaciens. Several transformants...
Potato cultivar Pito was transformed with the P1 sequence of potato virus Y (PVYO) in antisense orientation. Five transgenic lines (ASR) showed a high level of resistance to PVYO on mechanical and side-graft inoculation, expressed as no symptoms, and no detectable amounts of PVY in the inoculated and the upper uninoculated leaves, as determined by ELISA 21 and 35 days after inoculation. The possibility...
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