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Iron deficiency chlorosis is a significant yield-limiting problem in apple production, and screening or breeding for iron deficiency tolerant rootstocks is one of the best solutions. To increase the efficiency of selection for iron deficiency tolerant hybrids, a reverse northern blot-based dot-blot macroarray protocol was established in this paper. Using this macroarray, the expression levels...
Passiflora edulis Sims, which is native to South America, stands out as a passion fruit species with major potential for fruit production and marketing. This species is popularly known as yellow or purple passion fruit, depending on the color of the fruits produced. Brazil is the major worldwide producer of passion fruit; however, the average productivity of the country is low compared...
The aim of this study was to use a multi-environment trial approach from a mixed model point of view for factor analysis (FA) of the stability and adaptability of hybrids. Twenty-eight hybrids were analyzed in 35 environments across four seasons/years (summer season 2010, winter season 2011, summer season 2011 and winter season 2012). Several of these hybrids were analyzed during the first seasons...
Hop downy mildew (DM) is an obligate parasite causing severe losses in hop if not controlled. Resistance to this pathogen is a primary goal for hop breeding programs. The objective of this study was to identify QTLs linked to DM resistance. Next-generation-sequencing was performed on a mapping population segregating for DM resistance levels. Cloned plants were grown in a RCBD with three replicates...
The objective of this study was to agronomically characterize plants from induced chromosome doubling experiments in the Pisang Lilin parthenocarpic banana accession to select genotypes that will be used as 2n gamete donors in crosses with improved diploids to generate secondary triploids. Twenty-two plants were evaluated, of which 17 were autotetraploids. Compared with the original diploids, the...
Seed longevity is a crucial issue for germplasm conservation and seed marketing. This trait is determined not only by environmental conditions, but also by genetic factors. Molecular mapping of responsible loci has been performed with several crops, but not with tobacco ( Nicotianatabacum L.). In the present study, we investigate 122 recombinant inbred lines derived from...
Twenty five populations of Rauwolfia serpentina collected from different states of India were subjected to spectrophotometric and high performance liquid chromatography analyses to study magnitude of variation in total alkaloid and reserpine in roots. Significant variations in the content of total alkaloid (4.82–2.51 %) and reserpine (0.083–0.040 %) have been recorded. The total...
Wheat ( Triticum aestivum L.) has been widely grown as a winter forage crop in the southern Great Plains. However, development of wheat cultivars for winter forage production has not been a major goal of wheat breeding programs in the USA. Also, little information on forage yield and nutritive value of currently grown wheat varieties is available. The objective of this study was...
How many test locations and replications are needed in crop variety trials is a question every plant breeder has to ask. Simple formulas were developed to estimate the optimum number of replicates and test locations. The optimum number of replicates in a trial was estimated by the formula $$ {\text{N}}_{{\text{r}}} {\text{ = 3}}\left( {\sigma _{\epsilon }^{{\text{2}}} /\sigma _{{\text{g}}}^{{\text{2}}}...
Lycopene content is an important factor for determining watermelon fruit quality. However, the low DNA polymorphism among cultivated watermelon ( Citrullus lanatus ) has hindered the ability to establish high quality genetic maps and study the quantitative trait loci (QTL) controlling the lycopene content trait. In this study, we successfully constructed a genetic map of watermelon...
Branching is an important horticultural trait that influences plant architecture and production of cut chrysanthemum; however, its genetic determinism is poorly understood. In this study, an F 1 mapping population derived from a cross between two spray cut chrysanthemum cultivars was used to determine QTL underlying branching. The branching traits in the F 1 mapping...
Epistasis refers to the phenotypic effects of interactions between alleles of different loci. Statistical detection of such interactions remains to be the subject of presented studies. A method based on mixed linear model was developed for mapping QTLs with digenic epistasis. Reliable estimates of QTL additive and epistasis effects can be obtained by the maximum-likelihood estimate methods...
Genetically-modified coffee clones (GMCs) were presented in a previous work. They were created from a single commercial clone of Coffea canephora Pierre (clone 126). Therefore they all have the same genotype, except for the localization of the transgenic insertions. They synthesize the Bacillus thuringiensis endotoxin Cry1Ac against Leucoptera coffeella Guérin-Méneville,...
We investigated the effect of tissue source selection in combination with ion beam irradiation on generating flower-color mutants in Chrysanthemum morifolium . Flower color mutation in the chrysanthemum cultivar YoMystery (purple flowers) was used as an index to estimate mutation frequency and the spectrum of mutations. Somaclones regenerated from petal and leaf tissues and ion-beam...
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